abstract：:The aim of the study was to establish a murine model for sensitive screening of potential compounds with in vitro anti-influenza A virus activity. The evaluation in this in vivo model is based on semi-quantitative detection of viral RNA using one-step reverse transcriptase polymerase chain reaction (RT-PCR). After int...

journal_title：Antiviral research

authors： Sauerbrei A,Ulbricht A,Wutzler P

更新日期：2003-03-01 00:00:00

abstract：:The era of antiviral chemotherapy started more than 50 years with the findings by Domagk and his colleagues that thiosemicarbazones showed activity against vaccinia virus. One of the derivatives, methisazone, was even investigated in the prophylaxis of smallpox. With the successful implementation of the smallpox vacci...

journal_title：Antiviral research

authors： Neyts J,De Clercq E

更新日期：2003-01-01 00:00:00

abstract：:Influenza A virus has been responsible for widespread human epidemics because it is readily transmitted from humans to humans by aerosol. Recent events have highlighted the potential of influenza A virus as a bioterrorist weapon: the high virulence of the influenza A virus that infected people in Hong Kong in 1997; an...

journal_title：Antiviral research

authors： Krug RM

更新日期：2003-01-01 00:00:00

abstract：:Emergence of drug-resistant human cytomegalovirus (HCMV) strains is a substantial problem during treatment of HCMV infections in immunocompromised patients. The Z-isomers of 2-hydroxymethylcyclopropylidenemethyl adenine (synadenol) and guanine (synguanol) were previously shown to be potent inhibitors of AD169 and Town...

journal_title：Antiviral research

authors： Baldanti F,Sarasini A,Drach JC,Zemlicka J,Gerna G

更新日期：2002-12-01 00:00:00

abstract：:In cultured mammalian cells (Vero), different antiviral agents change to differing degrees the ability of HSV2 to down-regulate gap junctions, each agent having a specific effect. Measured by intracellular electrodes, control cell populations showed 49-51% coupling, uninfected populations treated with acyclovir or SDS...

journal_title：Antiviral research

authors： Musée J,Mbuy GN,Woodruff RI

更新日期：2002-11-01 00:00:00

abstract：:This study describes the extent of cross-resistance and interactions for selected inhibitors of human cytomegalovirus (HCMV) DNA synthesis and DNA processing. HCMV isolates resistant to the benzimidazole D-ribonucleoside viral DNA processing inhibitors TCRB and BDCRB were sensitive to BAY 38-4766, a non-nucleoside inh...

journal_title：Antiviral research

authors： Evers DL,Komazin G,Shin D,Hwang DD,Townsend LB,Drach JC

更新日期：2002-10-01 00:00:00

abstract：:Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...

journal_title：Antiviral research

authors： Ouzounov S,Mehta A,Dwek RA,Block TM,Jordan R

更新日期：2002-09-01 00:00:00

abstract：:Herpesviruses encode a protease that is essential for virus replication. The protease undergoes cleavage to a processed form during capsid maturation. A recombinant 75 kDa form of the protease from human cytomegalovirus was purified and compared with the recombinant 29 kDa processed form. Modification with an active s...

journal_title：Antiviral research

authors： Wittwer AJ,Funckes-Shippy CL,Hippenmeyer PJ

更新日期：2002-08-01 00:00:00

abstract：:Mycophenolate mofetil (MMF), a therapeutically used inhibitor of inosine monophosphate dehydrogenase is hydrolyzed to its active metabolite mycophenolic acid (MPA) in vivo. MPA exhibits anti-HIV activity in vitro. We tested MPA alone and in combination with abacavir (ABC), didanosine (DDI), lamivudine (3TC) and tenofo...

journal_title：Antiviral research

authors： Hossain MM,Coull JJ,Drusano GL,Margolis DM

更新日期：2002-07-01 00:00:00

abstract：:In the scope of the search for new anti-HIV agents interacting with a new target, we developed a high-throughput screening system to detect the interactions between Nef and Hck. Nef is an accessory protein of HIV, which is involved in the pathogenicity of the acquired immunodeficiency syndrome (AIDS). Nef is also a si...

journal_title：Antiviral research

authors： Murakami Y,Fukazawa H,Kobatake T,Yamagoe S,Takebe Y,Tobiume M,Matsuda M,Uehara Y

更新日期：2002-07-01 00:00:00

abstract：:Because encapsulation of antiviral drugs in liposomes resulted generally in improved activity against retroviral replication in vivo, the antiviral effects of free-SPC3 and liposome-associated SPC3 were compared in cultured human lymphocytes infected with HIV-1. SPC3 was entrapped in various liposomal formulations, ei...

journal_title：Antiviral research

authors： de Mareuil J,Mabrouk K,Doria E,Moulard M,de Chasteigner S,Oughideni R,van Rietschoten J,Rochat H,De Waard M,Sabatier JM

更新日期：2002-06-01 00:00:00

abstract：:The biological evaluation of mononucleotide prodrugs (pronucleotides) of various nucleoside reverse transcriptase inhibitors (NRTIs) such as zidovudine (AZT), zalcitabine (ddC) and lamivudine (3TC) was reported in human T-lymphoid MOLT-4/8 cells which were grown continuously for more than 1 year in a medium containing...

journal_title：Antiviral research

authors： Gröschel B,Cinatl J,Périgaud C,Gosselin G,Imbach JL,Doerr HW,Cinatl J Jr

更新日期：2002-02-01 00:00:00

abstract：:Lactoferrin (LF) is an iron binding glycoprotein that is present in several mucosal secretions. Many biological functions have been ascribed to LF. One of the functions of LF is the transport of metals, but LF is also an important component of the non-specific immune system, since LF has antimicrobial properties again...

journal_title：Antiviral research

authors： van der Strate BW,Beljaars L,Molema G,Harmsen MC,Meijer DK

更新日期：2001-12-01 00:00:00

abstract：:Infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) may result in a number of different clinical outcomes. There is strong evidence in HBV infection that host genetic factors play a major role in determining the outcome of infection. A number of approaches may be used to determine the specific genetic fa...

journal_title：Antiviral research

authors： Thursz M

更新日期：2001-11-01 00:00:00

abstract：:To compare incidence, risk factors and morphologic pattern of hepatocellular carcinoma (HCC) development in hepatitis B virus (HBV) and hepatitis C virus (HCV) related cirrhosis, 401 patients were followed prospectively by periodic ultrasound examination for 14-189 months (mean: 84.8+/-36.7). During follow-up, 77 (19....

journal_title：Antiviral research

authors： Benvegnù L,Alberti A

更新日期：2001-11-01 00:00:00

abstract：:Although highly active anti-retroviral therapy (HAART) is successful in the treatment of HIV infection, problems with toxicity, drug-resistant variants, and therapeutic failures have compromised the long-term utility of existing combination regimens. Mismatched double-stranded RNA (polyI-polyC(12)U) is an immune modul...

journal_title：Antiviral research

authors： Essey RJ,McDougall BR,Robinson WE Jr

更新日期：2001-09-01 00:00:00

abstract：:The replication cycle of the human cytomegalovirus (HCMV) is characterized by the expression of immediate early (IE), early (E), and late (L) gene regions. Current antiviral strategies are directed against the viral DNA polymerase expressed during the early phase of infection. The regulation of the IE-1 and IE-2 gene ...

journal_title：Antiviral research

authors： Scholz M,Doerr HW,Cinatl J

更新日期：2001-03-01 00:00:00

abstract：BACKGROUND AND AIMS:Interferon-alpha treatment has been the treatment of choice for chronic hepatitis with unpredictable results. Recently, Lamivudine has been licensed for use against HBV infection with good results. Unfortunately, recurrence of viremia after lamivudine withdrawal is common and prolonged treatment can...

journal_title：Antiviral research

authors： Andreone P,Fiorino S,Cursaro C,Gramenzi A,Margotti M,Di Giammarino L,Biselli M,Miniero R,Gasbarrini G,Bernardi M

更新日期：2001-02-01 00:00:00

abstract：:DNA immunizations with the major structural protein VP1 of Coxsackie virus B3 (CVB3) have been previously found to protect BALB/c mice from lethal challenge. Here we report that the other CVB3 capsid proteins, VP2, VP3, and VP4, were less effective at preventing CVB3-caused disease. The application of pCMV/VP1 as a va...

journal_title：Antiviral research

authors： Henke A,Zell R,Stelzner A

更新日期：2001-01-01 00:00:00

abstract：:Innate, non-specific, resistance mechanisms are important barriers to pathogens, particularly delaying virus multiplication at the onset of infections. These innate defense mechanisms include a series of mechanical barriers, pre-existing inhibitory molecules, and cellular responses with antimicrobial activity. The ant...

journal_title：Antiviral research

authors： Baron S,Singh I,Chopra A,Coppenhaver D,Pan J

更新日期：2000-11-01 00:00:00

abstract：:We have analyzed a panel of protein kinase inhibitors (PKIs) and found that some indolocarbazoles (Gö6976, K252a, K252c) proved to be highly effective inhibitors of GCV-sensitive and -resistant human cytomegalovirus (HCMV) strains, but did not show any effect against herpes simplex virus. Antiviral activity was determ...

journal_title：Antiviral research

authors： Zimmermann A,Wilts H,Lenhardt M,Hahn M,Mertens T

更新日期：2000-10-01 00:00:00

abstract：:Salvage therapy with ritonavir (RTV) and saquinavir (SQV) failed to achieve virological and immunological improvement in 24 HIV-infected patients who discontinued triple therapy with RTV or indinavir (IDV) because of failure or intolerance to treatment. Changes in the HIV-1 protease gene sequence were analyzed prospec...

journal_title：Antiviral research

authors： Karmochkine M,Si Mohamed A,Piketty C,Ginsburg C,Raguin G,Schneider-Fauveau V,Gutmann L,Kazatchkine MD,Belec L

更新日期：2000-09-01 00:00:00

abstract：:AIDS therapies employing HIV protease inhibitors (PIs) are associated with changes in fat metabolism. However, the cellular mechanisms affected by PIs are not clear. Thus, the affects of PIs on adipocyte differentiation were examined in vitro using C3H10T1/2 stem cells. In these cells the PIs, nelfinavir, saquinavir, ...

journal_title：Antiviral research

authors： Lenhard JM,Furfine ES,Jain RG,Ittoop O,Orband-Miller LA,Blanchard SG,Paulik MA,Weiel JE

更新日期：2000-08-01 00:00:00

abstract：:Combinations of anti-HIV agents including one or two reverse transcriptase inhibitors with a protease inhibitor are potent and effective. However, toxicities, costs and the emergence of drug-resistant organisms have compromised their long-term efficacy in people. A next, likely, target for anti-HIV therapy is HIV-1 in...

journal_title：Antiviral research

authors： Beale KK,Robinson WE Jr

更新日期：2000-06-01 00:00:00

abstract：:A fatty acid derivative of ganciclovir (GCV), elaidic acid ganciclovir (E-GCV), has been evaluated for its inhibitory activity against laboratory and clinical strains of herpes simplex type 1 (HSV-1) and type 2 (HSV-2), varicella-zoster virus (VZV) and human cytomegalovirus (HCMV) in human embryonic lung fibroblasts. ...

journal_title：Antiviral research

authors： Andrei G,Snoeck R,Neyts J,Sandvold ML,Myhren F,De Clercq E

更新日期：2000-03-01 00:00:00

abstract：:Previously, we found that a ganciclovir (GCV)-resistant clinical human cytomegalovirus (HCMV) isolate had an amino acid substitution at codon 501 (Leu --> Phe) in the delta-region C of the DNA polymerase gene. DNA polymerases have now been (partially) purified from both the GCV-resistant and sensitive parental strains...

journal_title：Antiviral research

authors： Kariya M,Mori S,Eizuru Y

更新日期：2000-02-01 00:00:00

abstract：:The effects of HIV-1 protease inhibitors on proteolytic processing and infectivity of virions produced from lymphocytes chronically infected with the virus were studied. Protease inhibition was detected by the accumulation of the polyprotein precursors Pr55gag and Pr160gag-pol and their cleavage intermediates. Immunob...

journal_title：Antiviral research

authors： Jardine DK,Tyssen DP,Birch CJ

更新日期：2000-01-01 00:00:00

abstract：:Using several herbal extracts, we investigated whether certain Kampo medicines exert an inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS), and thereby inhibit the growth of influenza A virus in Madin-Darby canine kidney cells. The vital fluorescen...

journal_title：Antiviral research

authors： Mantani N,Andoh T,Kawamata H,Terasawa K,Ochiai H

更新日期：1999-12-31 00:00:00

abstract：:The antiviral effect of Hochu-ekki-to (TJ-41), a Japanese herbal medicine, was investigated using mice infected with influenza virus. TJ-41 was found to increase the survival rate, prolong the mean survival days, suppress viral growth in bronchoalveolar labage fluid (BALF) and inhibit the lung index (lung consolidatio...

journal_title：Antiviral research

authors： Mori K,Kido T,Daikuhara H,Sakakibara I,Sakata T,Shimizu K,Amagaya S,Sasaki H,Komatsu Y

更新日期：1999-12-15 00:00:00

abstract：:Chronic hepatitis B infection remains a major public health problem worldwide. The hepatitis B virus belongs to the family of hepadnaviruses that replicate their DNA genome via a reverse transcription pathway. The chronicity of infection in infected hepatocytes is maintained by the persistence of the viral covalently ...

journal_title：Antiviral research

authors： Zoulim F

更新日期：1999-11-01 00:00:00

abstract：:Isoborneol, a monoterpene and a component of several plant essential oils, showed dual viricidal activity against herpes simplex virus 1 (HSV-1). First, it inactivated HSV-1 by almost 4 log10 values within 30 min of exposure, and second, isoborneol at a concentration of 0.06% completely inhibited viral replication, wi...

journal_title：Antiviral research

authors： Armaka M,Papanikolaou E,Sivropoulou A,Arsenakis M

更新日期：1999-09-01 00:00:00

abstract：:This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) an...

journal_title：Antiviral research

authors： McSharry JJ

更新日期：1999-08-01 00:00:00

abstract：:HIV-host infection systems in vitro are important in the pre-clinical assessment of anti-retroviral drug activity. The present report describes the development of a new HIV-host model comprised of an epithelial cell line of HeLa lineage (HeLa-1), transfected with expression vectors bearing tat and rev (TART) genes of ...

journal_title：Antiviral research

authors： Moussazadeh M,Hua J,Sidhu MK,Zhao XX,Fitzgibbon JE,Liao MJ,Rashidbaigi A

更新日期：1999-07-01 00:00:00

abstract：:In an ongoing effort to identify an orally bioavailable compound for the treatment of rhino- and enteroviral infections, a series of vinylacetylene benzimidazoles was recently examined. Previous studies demonstrated the potential for these compounds to possess both good in vitro antiviral activity as well as acceptabl...

journal_title：Antiviral research

authors： Tebbe MJ,Jensen CB,Spitzer WA,Franklin RB,George MC,Phillips DL

更新日期：1999-05-01 00:00:00

abstract：:Despite an urgent medical need, a broadly effective anti-viral therapy for the treatment of infections with hepatitis C viruses (HCVs) has yet to be developed. One of the approaches to anti-HCV drug discovery is the design and development of specific small molecule drugs to inhibit the proteolytic processing of the HC...

journal_title：Antiviral research

authors： Kwong AD,Kim JL,Rao G,Lipovsek D,Raybuck SA

更新日期：1999-02-01 00:00:00

abstract：:We have characterized the differential actions of acyclovir and penciclovir against varicella-zoster virus (VZV) in cell culture by comparing the frequency of appearance of resistant viruses followed by their characterization. Cells were infected with cell-free virus and the cultures were successively treated with inc...

journal_title：Antiviral research

authors： Ida M,Kageyama S,Sato H,Kamiyama T,Yamamura J,Kurokawa M,Morohashi M,Shiraki K

更新日期：1999-01-01 00:00:00

abstract：:We isolated a ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) from a laboratory strain, AD169, and analysed the mutant. Attempts were also made to identify directly the mutated gene. The 50% inhibitory concentration (IC50) of GCV for the mutant strain was five times higher than that of the wild-type strain. T...

journal_title：Antiviral research

authors： Faizi Khan R,Mori S,Eizuru Y,Kumura Ishii K,Minamishima Y

更新日期：1998-12-01 00:00:00

abstract：:Viracea, a topical microbicide, is a blend of benzalkonium chloride and phytochemicals derived from Echinacea purpurea and is a proprietary formula from Destiny BioMediX Corp. Viracea was tested against 40 strains of herpes simplex virus (HSV): 15 strains (five HSV-1 and ten HSV-2) were resistant to acyclovir (ACV-R) ...

journal_title：Antiviral research

authors： Thompson KD

更新日期：1998-07-01 00:00:00

abstract：:Amantadine and rimantadine hydrochloride were tested for stability after storage at different temperatures and under different conditions for extended periods of time. Both compounds were quite stable after storage for at least 25 years at ambient temperature; they both retained full antiviral activity after long-term...

journal_title：Antiviral research

authors： Scholtissek C,Webster RG

更新日期：1998-06-01 00:00:00

abstract：:Introduction of segmented genomes into virion is an important process in viral replication of rotavirus. We previously studied the assortment of the VP7 gene segment (encoding outer capsid protein VP7) in the genetic background of simian rotavirus SA11 (G serotype 3, G3) and found the preferential selection of homolog...

journal_title：Antiviral research

authors： Okada J,Kobayashi N,Taniguchi K,Urasawa S

更新日期：1998-04-01 00:00:00